147.14—Procedures to determine status and effectiveness of sanitation monitored program.
The following monitoring procedures 10 may be applied at the discretion of the Official State Agency:
Code of Federal Regulations
Footnote(s): 10 Laboratory procedures for monitoring operations proposed here are described in the following two publications: Isolation and Identification of Avian Pathogens, American Association of Avian Pathologists, University of Pennsylvania, New Bolton Center, Kennett Square, Pennsylvania 19348-1692, 1980, and Culture Methods for the Detection of Animal Salmonellosis and Arizonosis, Iowa State University Press, Ames, Iowa 50010, 1976.
(1)
Culture the surface of cased eggs periodically for fecal contaminating organisms as described in § 147.13.
(2)
Culture a sample of dead-in-shell eggs periodically from each breeding flock for coliforms. Such eggs should also be cultured for the dependable recovery of salmonellae. Culturing for the dependable recovery of salmonellae should include the use of:
(i)
Preenrichment broths supplemented with 35 mg ferrous sulfate per 1,000 ml preenrichment to block iron-binding, Salmonella -inhibiting effects of egg conalbumin; and
(ii)
Tetrathionate selective enrichment broths, competitor-controlling plating media (XLT4, BGN, etc.), delayed secondary enrichment procedures, and colony lift assays detailed in paragraph (a)(5) and illustration 2 of § 147.11.