CFR > Title 9 - Animals and Animal Products > CHAPTER I—ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE > SUBCHAPTER E—VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS (parts 101 to 124) > PART 113—STANDARD REQUIREMENTS > 113.32—Detection of Brucella contamination.

113.32—Detection of Brucella contamination.

The test for detection of Brucella contamination provided in this section shall be conducted when such a test is prescribed in an applicable Standard Requirement or in a filed Outline of Production for the product.
(a) One ml of the minced tissue used as the source of cells or 1 ml of the extract of the tissue prior to the addition of antibiotics, diluent and stabilizer, shall be inoculated onto each of three tryptose agar plates and incubated in a 10 percent CO2 atmosphere at a temperature of 35-37 °C for at least 7 days.
(b) If colonies are identified as Brucella, the biological product is unsatisfactory.
(c) If colonies suspicious of Brucella are observed but cannot be identified as a Brucella species, either
(1) The biological product shall be regarded as unsatisfactory and destroyed; or
(2) Further subculture or other procedures shall be carried out until a positive identification can be made.

Code of Federal Regulations

[38 FR 29888, Oct. 30, 1973]